SpeciesReactivity
Mouse

Form
Stds=10-200 ng/ml; Sample=100 ul (diluted); Sensitivity= 10 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Anti-Mouse IgM coated strip plate (8 wells x 12 strips) 6381 2. Mouse IgM Std. A (0 ng/ml), 0.25 ml 6382a 3. Mouse IgM Std. B (50 ng/ml), 0.25 ml 6382b 4. Mouse IgM Std. C (125 ng/ml), 0.25 ml 6382c 5. Mouse IgM Std. D (250 ng/ml), 0. 25 ml 6382d 6. Mouse IgM Std. E (500 ng/ml), 0. 25 ml 6382e 7. Mouse IgM Std. F (1000 ng/ml), 0. 25 ml 6382f 8. Wash Buffer (100X), 10 ml WB-100 9. Sample Diluent (20x), 10ml SD-20 10. Anti-Mouse IgM HRP Conjugate (100x), 0.12 ml 6383 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-6380

SpCrossreactivity
The antibodies used in this kit are specific for IgM with no significant reactivity to IgG1-3, or IgA, IgE. Mouse IgM ELISA kit species crossreactivity was tested with the following animal sera (IgM) at dilutions of 1:400: Significant crossreactivity was observed with Rat (67%), and Hamster (52%) serum IgM. Guinea Pig, Rabbit, Human, Monkey, Bovine, FBS, Goat, Sheep, Pig, and Chicken serum IgM had no significant crossreactivity.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.