SpeciesReactivity
Human

Form
Stds=0-100 ng/ml; Sample=25 ul; Sensitivity=0.2 ng/ml; 150 min assay; Quantitative; Competitive assay; 96 tests

Samples
Serum. Other fluids not optimized.

KitContents
1. Anti-neopterin antibody coated Strip plate, (96 wells) 34112. Neopterin Std. A, 1 ml, 0 ng/ml 3412A3. Neopterin Std. B, 0.5 ml; 0.5 ng/ml 3412B4. Neopterin Std. C, 0.5 ml; 1.5 ng/ml 3412C5. Neopterin Std. D, 0.5 ml; 3.0 ng/ml 3412D6. Neopterin Std. E, 0.5 ml; 6.0 ng/ml 3412E7. Neopterin Std. F, 0.5 ml; 12.0 ng/ml 3412F8. Neopterin Std. G, 0.5 ml; 24.0 ng/ml 3412G9. Neopterin Std. H, 0.5 ml; 100.0 ng/ml 3412H10. Neopterin Control 1, 0.5 ml (values on the vial) 3413C111. Neopterin Control 2, 0.5 ml (values on the vial) 3413C212. Stds and Controls prepared human serum base containing 0.02% thimerosal 13. Neopterin Conjugate Diluent, 18 ml 341414. Neopterin-HRP Conjugate, 0.3 ml (100X) 341515. Wash buffer 10 ml (10X) WN-1016. TMB Substrate Solution, 11 ml TMB-2S17. Stop Solution, 11 ml ST-341018. Complete Instruction Manual M-3410

SpCrossreactivity
Following relevant substances were tested for cross Reactivity: 7,8-Dihydro-Neopterin (3.5%), Monapterin, Biopterin, Dihydro-D-Biopterin, Tetrahydro-Neopterin, and Xanthopterin (<0.3%). This kit has been designed and tested for human samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since neopterin is the same in all species, this kit should work in most species as long as the sample cocn is within the range of this kit.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.