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當前位置:首頁技術文章Human Sonic Hedgehog

Human Sonic Hedgehog

更新時間:2013-06-17點擊次數:1243

Synonyms :
Shh, HHG-1
Description :
Hedgehog (Hh) proteins are widely distributed throughout the animal kingdom and play important regulatory roles in various development processes. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino acid sequence identity (e.g. Shh and Ihh are 93 % identical). Although, Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they signal through the same Patched (Ptc)/Smoothened (Smo) receptor complex, activate the same intracellular signaling pathway, and can substitute for each other in experimental systems. The mature biologically active form of Hh molecules is produced by autocatalytic cleavage of their precursor proteins and corresponds to approximay the N-terminal half of the precursor molecule. Recombinant human Shh is a 20.6 kDa protein consisting of 174 amino acid residues, which correspond to the active N-terminal portion of the Shh precursor.
Catalog #:
100-45B
Source :
(BTI-Tn-5B1-4) High-5 Insect Cells*
Stability :
The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted Shh is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.3
Purity :
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin Level :
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Biological Activity :
Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.
PubMed Link :
Sonic+AND+Hedgehog
Country of Origin :
USA
 

 
 
Size A
2µg
$75 
 
 
Size B
10µg
$185 
 
 
 

 
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